Prolactin actions in the mouse mediobasal hypothalamus (#242)
Male C57BL/6 mice were treated with bromocriptine over 24 h in order to block endogenous prolactin release. Intraperitoneal injections of 2mg/kg ovine prolactin were administered over 2, 5, 20 or 60 min. Animals were euthanised with pentobarbital and perfused with 4% paraformaldehyde. Brains were excised and cryostat sections prepared. Phospho-STAT5 staining was visualised through immunohistochemical techniques. Phospho-STAT5 in the arcuate nucleus and ME was counted, where nuclear, or analysed using densometric measures where apparent in a punctate form. The results show a temporal change in the nuclear staining observed in the arcuate nucleus, with immediate responses at 2 min (p<0.005), increasing by four-fold at 60 min. In the ME, the response visible at 2 min was three-fold the saline response (p<0.05), but remained at this level across the time course. This data demonstrates the responses of the ME are temporally distinct to that observed in the arcuate nucleus suggesting a possible functional separation